性能参数

产品名称

3BDO

规格

10mM*1mLinDMSO 5mg 10mg 25mg

货号

GOY-Y1862

 含量

>98.00%

CAS

890405-51-3

别名

N/A

化学名

N/A

分子式

C18H17NO5

分子量

分子 327.33

溶解度

DMSO : ≥ 150 mg/mL (458.25 mM)

储存条件

Store at -20°C

General tips

用途

仅供科研

价格

电询

详细内容

3BDO is a new mTOR activator which can also inhibit autophagy.

Phosphorylation of RPS6KB1 and EIF4EBP1 is significantly increased by 3BDO with vector alone but suppressed with FKBP1A overexpression. Rapamycin fails to decrease the phosphorylation of MTOR and RPS6KB1 in the presence of 3BDO. 3BDO suppresses the increase in MAP1LC3B puncta induced with rapamycin. 3BDO also inhibits the effect of rapamycin in HUVECs. The phosphorylation of Ser residues is decreased in HUVECs treated with 10 μM rapamycin, and 60 μM 3BDO reverses the phosphorylation. The results show that 3BDO suppresses the increased MAP1LC3B puncta number, MAP1LC3B-II level and decreased SQSTM1 protein level induced by rapamycin. 3BDO could dose- and time-dependently decrease FLJ11812 level in HUVECs. Overexpression of FLJ11812 reverses the inhibition of autophagy induced by 3BDO[1].

Immunofluorescence assay reveals that 3BDO treatment increases the level of p-p70S6K and decreases the protein level of ATG13 in plaque endothelium of mice. 3BDO does not affect the phosphorylation of mTOR direct downstream targets p70S6K and 4EBP1. As compare with controls, apoE-/- mice show inhibited endothelium autophagy and apoptosis with 3BDO treatment, so 3BDO protects against endothelium injury in atherosclerosis. 3BDO treatment stabilizes established atherosclerotic lesions in apoE-/- mice. In apoE-/-mice, as compare with controls, with 3BDO treatment, the serum level of IL-6 and IL-8 is significantly decreased[2].

[1]. Ge D, et al. Identification of a novel MTOR activator and discovery of a competing endogenous RNA regulating autophagy in vascular endothelial cells. Autophagy. 2014 Jun;10(6):957-71. [2]. Peng N, et al. An activator of mTOR inhibits oxLDL-induced autophagy and apoptosis in vascular endothelial cells and restricts atherosclerosis in apolipoprotein E?/? mice. Sci Rep. 2014 Jul 1;4:5519.

D-别异亮氨酸 98%  水胺硫磷标准溶液 10μg/ml,u=5～3%，丙酮（剧毒品）

Fmoc-Ile-OPfp 98%  水胺硫磷标准溶液 100μg/ml,u=5～3%，丙酮（剧毒品）

H-Ile-2-Chlorotrityl Resin 0.3~0.8 mmol/g, 100~200 mesh  亚胺硫磷标准溶液 10μg/ml,u=6～2%，丙酮

Fmoc-Ile-Wang resin 100-200 mesh, Substitution 0.3-0.8mmol/g  亚胺硫磷标准溶液 100?

EHMT2-IN-110mM*1mL in DMSO 5mg 10mg 25mg>98.00%Cas No. 2230849-55-3C18H23N7O

E74491mg  5mg 10mg 25mg>98.00%Cas No. 1140964-99-3C18H15N5O

E-73861mg  5mg 10mg 50mg>98.00%Cas No. 1799824-08-0C39H48FN9O4

EPZ0316861mg  5mg 10mg 50mg>98.00%Cas No. 1808011-22-4C26H34ClF3N4O4S

EPZ011989 trifluoroacetate (EPZ-011989 trifluoroacetate)5mg  10mg 50mg 100mg>98.00%Cas No. 1598383-41-5C37H52F3N5O6

EPZ0119895mg  10mg 50mg 100mg>98.00%Cas No. 1598383-40-4C35H51N5O N/AC28H41N7O4·CH2O2